https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49765 Wed 31 May 2023 09:27:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50289 Wed 28 Feb 2024 16:38:42 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52402 Wed 28 Feb 2024 15:53:13 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47871  0.5 and diabetes comorbidity were the most significant predictors of hypertension presence, awareness and treatment. Individuals with diabetes were twice as likely to have hypertension, 7.0 times more likely to be aware, 3.3 times more likely to be on antihypertensive medication, and 2.4 times more likely to be controlled on medication. Women and individuals reporting lower salt use were more likely to be aware and treated for hypertension. Applying the 2017 AHA/ACC hypertension guidelines showed only 1 in 4 adults had normal BP. As with HIV, similarly intensive efforts are now needed in the region to improve non-communicable disease diagnosis and management.]]> Wed 28 Feb 2024 14:59:16 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45264 Wed 26 Oct 2022 18:09:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24527 Wed 24 Nov 2021 15:50:30 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23155 Wed 24 Aug 2016 15:58:37 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37515 Wed 23 Aug 2023 09:36:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48511 n = 321,223) and offspring birth weight (n = 230,069 mothers), we identified 190 independent association signals (129 of which are novel). We used structural equation modeling to decompose the contributions of direct fetal and indirect maternal genetic effects, then applied Mendelian randomization to illuminate causal pathways. For example, both indirect maternal and direct fetal genetic effects drive the observational relationship between lower birth weight and higher later blood pressure: maternal blood pressure-raising alleles reduce offspring birth weight, but only direct fetal effects of these alleles, once inherited, increase later offspring blood pressure. Using maternal birth weight-lowering genotypes to proxy for an adverse intrauterine environment provided no evidence that it causally raises offspring blood pressure, indicating that the inverse birth weight-blood pressure association is attributable to genetic effects, and not to intrauterine programming.]]> Wed 22 Mar 2023 15:25:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23967 Wed 17 Nov 2021 16:30:14 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32982 Wed 17 Nov 2021 16:28:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30321 Wed 16 May 2018 13:39:09 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43022 Wed 15 May 2024 09:07:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33058 Wed 15 Dec 2021 16:09:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38675 0.05). The IBS-Quality of Life instrument showed moderate correlations with the FAST symptom abdominal swelling/distension (0.313-0.416, P < 0.05). The consumption of a high fermentable oligosaccharides, disaccharides, monosaccharides, and polyols meal was associated with participants with IBS-D experiencing abdominal bloating and participants with IBS-C not experiencing abdominal swelling (P < 0.05). The consumption of fiber was correlated with abdominal fullness and bloating in participants with IBS-C (P < 0.05). Discussion: The FAST diary validly measures gastrointestinal symptoms as they occur in people with IBS and correlates these symptoms with specific aspects of diet.]]> Wed 15 Dec 2021 15:35:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14264 −11) was with rs1625579 within an intron of a putative primary transcript for MIR137 (microRNA 137), a known regulator of neuronal development. Four other schizophrenia loci achieving genome-wide significance contain predicted targets of MIR137, suggesting MIR137-mediated dysregulation as a previously unknown etiologic mechanism in schizophrenia. In a joint analysis with a bipolar disorder sample (16,374 affected individuals and 14,044 controls), three loci reached genome-wide significance: CACNA1C (rs4765905, P = 7.0 × 10⁻⁹), ANK3 (rs10994359, P = 2.5 × 10⁻⁸) and the ITIH3-ITIH4 region (rs2239547, P = 7.8 × 10⁻⁹).]]> Wed 11 Apr 2018 18:45:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21727 EL by protein phosphatase 2A (PP2A). However, melanoma cells are largely resistant to ER stress-induced apoptosis, suggesting that Bim activation is suppressed in melanoma cells undergoing ER stress. We show here that ER stress reduces PP2A activity leading to increased ERK activation and subsequent phosphorylation and proteasomal degradation of Bim_EL. Despite sustained upregulation of Bim at the transcriptional level, the Bim_EL protein expression was downregulated after an initial increase in melanoma cells subjected to pharmacological ER stress. This was mediated by increased activity of ERK, whereas the phosphatase activity of PP2A was reduced by ER stress in melanoma cells. The increase in ERK activation was, at least in part, due to reduced dephosphorylation by PP2A, which was associated with downregulation of the PP2A catalytic C subunit. Notably, instead of direct dephosphorylation of Bim_EL, PP2A inhibited its phosphorylation indirectly through dephosphorylation of ERK in melanoma cells. Taken together, these results identify downregualtion of PP2A activity as an important protective mechanism of melanoma cells against ER stress-induced apoptosis.]]> Wed 11 Apr 2018 17:12:53 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22674 in-situ occlusive thrombus formation (Folt’s model of ‘physiological’ thrombus). Intravenous tPA was administered 60 minutes post-occlusion (n = 6-7/group). Sustained recanalization rates were 0%, 17%, 67% and 71%, for 0.9, 1.8, 4.5, and 10 mg/kg, respectively. Median time to sustained recanalization onset decreased with increasing dosage. We conclude that 10 mg/kg of tPA is too effective, whereas 0.9 mg/kg is ineffective for lysis of occlusive thrombi formed in situ. Neither dose mimics clinical tPA responses. A dose of 2x the clinical dose is a more appropriate mimic of clinical tPA recanalization in this model.]]> Wed 11 Apr 2018 16:44:50 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20924 Wed 11 Apr 2018 16:37:07 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24908 Wed 11 Apr 2018 13:53:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27558 Wed 11 Apr 2018 13:06:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25911 Wed 11 Apr 2018 12:36:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30816 80% activity vs. baseline. A significant, reversible, time-dependent inhibition was observed with lansoprazole (66% activity at 240 min, P = 0.034) and rabeprazole (25% activity at 240 min, P < 0.001). In regression analysis, PPI use was not associated with ADMA in HCS participants (beta 0.012, 95% CI −0.001 to 0.025, P = 0.077). Furthermore, there were no differences in ADMA between specific PPIs (P = 0.748). At clinical concentrations, PPIs are weak, reversible, DDAH1 inhibitors in vitro. The lack of significant associations between PPIs and ADMA in HCS participants questions the significance of DDAH1 inhibition as a mechanism explaining the increased cardiovascular risk reported with PPI use.]]> Wed 11 Apr 2018 12:32:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28242 Wed 11 Apr 2018 12:02:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30774 Wed 11 Apr 2018 11:53:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14353 Wed 11 Apr 2018 11:50:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14531 Wed 11 Apr 2018 11:18:53 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24878 Wed 11 Apr 2018 11:14:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15237 6 months after first- or second-line therapy were randomised to CD or CP for six cycles in this international, open-label, non-inferiority trial. The primary endpoint was progression-free survival. The OS analysis is presented here. Results: A total of 976 patients were randomised (467 to CD and 509 to CP). With a median follow-up of 49 months, no statistically significant difference was observed between arms in OS (hazard ratio=0.99 (95% confidence interval 0.85, 1.16); log-rank P=0.94). Median survival times were 30.7 months (CD) and 33.0 months (CP). No statistically significant difference in OS was observed between arms in predetermined subgroups according to age, body mass index, treatment-free interval, measurable disease, number of lines of prior chemotherapy, or performance status. Post-study cross-over was imbalanced between arms, with a greater proportion of patients randomised to CP receiving post-study PLD (68%) than patients randomised to CD receiving post-study paclitaxel (43%; P<0.001). Conclusion: Carboplatin-PLD led to delayed progression and similar OS compared with carboplatin-paclitaxel in platinum-sensitive ROC.]]> Wed 11 Apr 2018 10:14:17 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22707 BRCA₂, has a large number of genetic variants of unknown effect. The variant rs11571833, an A > T transversion in the final exon of the gene that leads to the creation of a stop codon 93 amino acids early (K3326*), is reported as a neutral polymorphism but there is some evidence to suggest an association with an increased risk of breast cancer. We assessed whether this variant was enriched in a cohort of breast cancer cases ascertained through familial cancer clinics compared to population-based non-cancer controls using a targeted sequencing approach. We identified the variant in 66/2634 (2.5%) cases and 33/1996 (1.65%) controls, indicating an enrichment in the breast cancer cases (p = 0.047, OR 1.53, 95% CI 1.00–2.34). This data is consistent with recent iCOGs data suggesting that this variant is not neutral with respect to breast cancer risk. rs11571833 may need to be included in SNP panels for evaluating breast cancer risk.]]> Wed 11 Apr 2018 09:55:17 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28216 Wed 11 Apr 2018 09:38:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36748 Wed 10 Nov 2021 15:04:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55109 Wed 10 Apr 2024 08:53:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25127 T (rs6917) polymorphism and the MTHFR 677 C>T (rs1801133) polymorphism, respectively. Results: There was no evidence of association between the PHB 1630 C>T and MTHFR 677 C>T polymorphisms with either disease for BRCA1 or BRCA2 mutation carriers when breast and ovarian cancer associations were evaluated separately. Analysis that evaluated associations for breast and ovarian cancer simultaneously showed some evidence that BRCA1 mutation carriers who had the rare homozygote genotype (TT) of the PHB 1630 C>T polymorphism were at increased risk of both breast and ovarian cancer (HR 1.50, 95%CI 1.10–2.04 and HR 2.16, 95%CI 1.24–3.76, respectively). However, there was no evidence of association under a multiplicative model for the effect of each minor allele. Conclusion: The PHB 1630TT genotype may modify breast and ovarian cancer risks in BRCA1 mutation carriers. This association need to be evaluated in larger series of BRCA1 mutation carriers.]]> Wed 07 Jul 2021 11:42:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34208 −8) in combined analyses. A number of these loci contain genes implicated in structure and function of skeletal muscle fibres (ACTG1), neuronal maintenance and signal transduction (PEX14, TGFA, SYT1), or monogenic syndromes with involvement of psychomotor impairment (PEX14, LRPPRC and KANSL1). Mendelian randomization analyses are consistent with a causal effect of higher genetically predicted grip strength on lower fracture risk. In conclusion, our findings provide new biological insight into the mechanistic underpinnings of grip strength and the causal role of muscular strength in age-related morbidities and mortality.]]> Wed 04 Sep 2019 09:48:47 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52160 Wed 04 Oct 2023 10:51:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41122 Tue 26 Jul 2022 14:31:29 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50437 Tue 25 Jul 2023 19:15:59 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50431 Tue 25 Jul 2023 19:01:27 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38873 Bacteroides species and describe the antimicrobial resistance biogeography along the intestine. We also detail which species, at which locations, are involved with the tryptophan/indole pathway, whose malfunctioning has been linked to pathologies including inflammatory bowel disease. Our study thus provides invaluable resources for investigating mechanisms connecting gut microbiota and host pathophysiology.]]> Tue 22 Feb 2022 16:36:25 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48301 Tue 14 Mar 2023 14:09:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51639 Tue 12 Sep 2023 20:15:00 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38579 in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy. The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin. The drug release process is divided into two stages: a rapid stage over the first 10 hr, then the release becomes gradual and stable. The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage. In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test. The LD₅₀ of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs. The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.]]> Tue 09 Nov 2021 15:27:06 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34657 Tue 03 Sep 2019 18:27:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38090 telomerase reverse transcriptase (TERT) have been associated with a wide range of cancers, including colorectal cancer (CRC) in LS. We combined genotype data from 1881 LS patients, carrying pathogenic variants in MLH1, MSH2 or MSH6, for rs2075786 (G>A, intronic variant), 1207 LS patients for rs2736108 (C>T, upstream variant) and 1201 LS patients for rs7705526 (C>A, intronic variant). The risk of cancer was estimated by heterozygous/homozygous odds ratio (OR) with mixed-effects logistic regression to adjust for gene/gender/country of sample origin considering family identity. The AA genotype of SNP rs2075786 is associated with 85% higher odds at developing cancer compared to GG genotype in MSH2 pathogenic variant carriers (p = 0.0160). Kaplan–Meier analysis also shows an association for rs2075786; the AA allele for MSH2 variant carriers confers risk for earlier diagnosis of LS cancer (log-rank p = 0.0011). We report a polymorphism in TERT to be a possible modifier of disease risk in MSH2 pathogenic variant carriers. The rs2075786 SNP in TERT is associated with a differential risk of developing cancer for MSH2 pathogenic variant carriers. Use of this information has the potential to personalise screening protocols for LS patients.]]> Tue 03 Aug 2021 19:10:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45347 Thu 27 Oct 2022 17:08:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45069 JMJD6 gene at 17q21-ter activates gene transcription. Here we show that JMJD6 forms protein complexes with N-Myc and BRD4, and is important for E2F2, N-Myc and c-Myc transcription. Knocking down JMJD6 reduces neuroblastoma cell proliferation and survival in vitro and tumor progression in mice, and high levels of JMJD6 expression in human neuroblastoma tissues independently predict poor patient prognosis. In addition, JMJD6 gene is associated with transcriptional super-enhancers. Combination therapy with the CDK7/super-enhancer inhibitor THZ1 and the histone deacetylase inhibitor panobinostat synergistically reduces JMJD6, E2F2, N-Myc, c-Myc expression, induces apoptosis in vitro and leads to neuroblastoma tumor regression in mice, which are significantly reversed by forced JMJD6 over-expression. Our findings therefore identify JMJD6 as a neuroblastoma tumorigenesis factor, and the combination therapy as a treatment strategy.]]> Thu 27 Oct 2022 14:03:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45314 Thu 27 Oct 2022 13:56:29 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50506 Thu 27 Jul 2023 13:10:52 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37758 regenerating islet-derived (REG) gene family are important regulators of many cellular processes. Here we functionally characterise a non-protein coding product of the family, the long noncoding RNA (lncRNA) REG1CP that is transcribed from a DNA fragment at the family locus previously thought to be a pseudogene. REG1CP forms an RNA–DNA triplex with a homopurine stretch at the distal promoter of the REG3A gene, through which the DNA helicase FANCJ is tethered to the core promoter of REG3A where it unwinds double stranded DNA and facilitates a permissive state for glucocorticoid receptor α (GRα)-mediated REG3A transcription. As such, REG1CP promotes cancer cell proliferation and tumorigenicity and its upregulation is associated with poor outcome of patients. REG1CP is also transcriptionally inducible by GRα, indicative of feedforward regulation. These results reveal the function and regulation of REG1CP and suggest that REG1CP may constitute a target for cancer treatment.]]> Thu 27 Jan 2022 15:55:02 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42206 Thu 25 Aug 2022 11:54:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35858 Thu 21 Oct 2021 12:45:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40967 Thu 21 Jul 2022 08:38:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35094 Thu 20 Jun 2019 15:56:20 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38442 Thu 18 Nov 2021 10:15:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35909 2 m/s), wave amplification and rapid changes in water level. Significant land inundation only occurs for scenarios modelled with the largest waves (9.0 M_W source). The degree of exposure to the open ocean and the geomorphology of locations within the Harbour determine the relative level of these impacts. Narrow, shallow channels, even those sheltered from the open ocean, create a bottleneck effect and experience the highest relative current speeds as well as elevated water levels. The largest maximum water levels (>8 m) occur in exposed, funnel-shaped bays and wave amplification is greatest at locations exposed to the open ocean: >7 times deep water wave heights for 9.0 M_W source waves. Upstream attenuation rates of runup and maximum water level show a linear correlation with wave height parameters at the 100 m depth contour and may provide some predictive capabilities for potential tsunami impacts at analogous locations. In the event of a tsunami in Sydney Harbour, impacts may threaten marine traffic and infrastructure.]]> Thu 16 Jan 2020 13:25:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34211 Thu 09 Dec 2021 11:02:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35835 Thu 03 Feb 2022 12:21:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7565 Sat 24 Mar 2018 08:42:03 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7421 Sat 24 Mar 2018 08:40:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8345 Sat 24 Mar 2018 08:39:53 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7942 Sat 24 Mar 2018 08:34:59 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7163 Sat 24 Mar 2018 08:34:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7162 Sat 24 Mar 2018 08:34:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7968 Sat 24 Mar 2018 08:33:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1976 Sat 24 Mar 2018 08:33:14 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1408 Sat 24 Mar 2018 08:28:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13695 1. Epidemiological studies have shown that genetic factors are primarily responsible for the substantially increased frequency of the disease seen in the relatives of affected individuals^2,3, and systematic attempts to identify linkage in multiplex families have confirmed that variation within the major histocompatibility complex (MHC) exerts the greatest individual effect on risk⁴. Modestly powered genome-wide association studies (GWAS)^5-10 have enabled more than 20 additional risk loci to be identified and have shown that multiple variants exerting modest individual effects have a key role in disease susceptibility¹¹. Most of the genetic architecture underlying susceptibility to the disease remains to be defined and is anticipated to require the analysis of sample sizes that are beyond the numbers currently available to individual research groups. In a collaborative GWAS involving 9,772 cases of European descent collected by 23 research groups working in 15 different countries, we have replicated almost all of the previously suggested associations and identified at least a further 29 novel susceptibility loci. Within the MHC we have refined the identity of the HLA-DRB1 risk alleles and confirmed that variation in the HLA-A gene underlies the independent protective effect attributable to the class I region. Immunologically relevant genes are significantly overrepresented among those mapping close to the identified loci and particularly implicate T-helper-cell differentiation in the pathogenesis of multiple sclerosis.]]> Sat 24 Mar 2018 08:19:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10937 1.0mm) (95%) and in metastatic melanoma in the skin (100%) and lymph node (81%). The immunoreactive score was highly related to progression of melanoma (P<0.0001). LDH5 expression was positively associated with increasing tumor thickness (P=0.02) and dermal tumor mitotic rate (P=0.02). LDH-5 above the median immunoreactive score was associated with reduced disease-free survival and overall survival (P<0.02). LDH-5 expression was negatively associated with Bcl-2 expression. In contrast, LDH-5 expression was strongly associated with Bcl-XL and Mcl-1 expression and also positively associated with GRP78 expression (P<0.0001). The low Bcl-2 expression in melanomas with high LDH-5 expression provides an explanation for the poor response of patients with high serum LDH levels to treatment with the Bcl-2 antisense drug ‘Genasense’. The strong correlation of LDH-5 expression with Mcl-1 expression suggests that treatment strategies inhibiting the activity of Mcl-1 in melanoma patients should be investigated.]]> Sat 24 Mar 2018 08:13:21 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10938 Sat 24 Mar 2018 08:13:21 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10550 Sat 24 Mar 2018 08:10:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10899 Sat 24 Mar 2018 08:09:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20536 Sat 24 Mar 2018 08:02:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21646 1-3 yet a large proportion of XLID cases remain unexplained, as each of the XLID genes identified so far only accounts for a small fraction (<1%) of affected individuals. Given that about one third of mutations affect gene expression levels,⁴ we reasoned that transcriptome profiling of lymphoblast cell lines from XLID patients may highlight genes harboring disease-causing mutations and may be an efficient follow-up method for rare sequence variants of unknown functional significance.]]> Sat 24 Mar 2018 07:52:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5036 Sat 24 Mar 2018 07:45:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5807 Sat 24 Mar 2018 07:44:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5001 Sat 24 Mar 2018 07:44:13 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30741 13CO₂ pulse labelling of ryegrass was used to monitor belowground C allocation, SOC priming, and stabilization of root-derived C for a 15-month period—commencing 8.2 years after biochar (Eucalyptus saligna, 550 °C) was amended into a subtropical ferralsol. We found that field-aged biochar enhanced the belowground recovery of new root-derived C (¹³C) by 20%, and facilitated negative rhizosphere priming (it slowed SOC mineralization by 5.5%, that is, 46 g CO₂-C m⁻² yr⁻¹). Retention of root-derived ¹³C in the stable organo-mineral fraction (<53 μm) was also increased (6%, P < 0.05). Through synchrotron-based spectroscopic analysis of bulk soil, field-aged biochar and microaggregates (<250 μm), we demonstrate that biochar accelerates the formation of microaggregates via organo-mineral interactions, resulting in the stabilization and accumulation of SOC in a rhodic ferralsol.]]> Sat 24 Mar 2018 07:39:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29439 Sat 24 Mar 2018 07:39:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27570 Sat 24 Mar 2018 07:23:30 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52520 Mon 29 Jan 2024 18:28:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44871 Mon 24 Oct 2022 11:16:35 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53956 Mon 22 Jan 2024 17:02:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33882 Thiobacillus and a novel group within the Oxalbacteraceae were enriched only on the MEBs and they had the genetic capacity for thiosulfate oxidation. All three surface-enriched bacteria also had the capacity to fix carbon dioxide, either in a potentially strictly autotrophic or mixotrophic manner. Our results show the dominance of chemolithotrophic processes on the surface of biochar and MEB that can contribute to carbon sequestration in soil.]]> Mon 21 Jan 2019 15:50:56 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35062 6+ doped Ni(OH)₂ nanosheet sample (w-Ni(OH)₂) with an outstanding oxygen evolution reaction (OER) performance that is, in a 1 M KOH medium, an overpotential of 237 mV is obtained reaching a current density of 10 mA/cm². Moreover, at high current density of 80 mA/cm², the overpotential value is 267 mV. The corresponding Tafel slope is measured to be 33 mV/dec. The d⁰ W⁶⁺ atom with a low spin-state has more outermost vacant orbitals, resulting in more water and OH- groups being adsorbed on the exposed W sites of the Ni(OH)₂ nanosheet. Density functional theory (DFT) calculations confirm that the O radical and O-O coupling are both generated at the same site of W⁶⁺. This work demonstrates that W⁶⁺ doping can promote the electrocatalytic water oxidation activity of Ni(OH)2 with the highest performance.]]> Mon 19 Aug 2019 16:37:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54840 Mon 18 Mar 2024 15:58:29 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35063 Malus domestica) carbohydrate metabolism, we cloned the coding sequences of MdFRK1 and MdFRK2 from the ‘Royal Gala’ apple. The results showed that MdFRK2 expression was extremely high in shoot tips and young fruit. Analyses of heterologously expressed proteins revealed that MdFRK2 had a higher affinity for fructose than did MdFRK1, with Km values of 0.1 and 0.62 mM for MdFRK2 and MdFRK1, respectively. The two proteins, however, exhibited similar Vmax values when their activities were significantly inhibited by high concentrations of fructose. MdFRK2 ectopic expression was associated with a general decrease in fructose concentration in transgenic lines. In leaves, increased FRK activity similarly resulted in reduced concentrations of glucose and sucrose but no alterations in sorbitol concentration. When compared with those in the untransformed control, genes involved in sorbitol synthesis (A6PR) and the degradation pathway (SDH1/2) were significantly upregulated in transgenic lines, whereas those involved in sucrose synthesis (SPS1) and other degradation processes (SUSY4, NINV1/2, and HxK2) were downregulated. The activity of enzymes participating in carbohydrate metabolism was proportional to the level of gene expression. However, the growth performance and photosynthetic efficiency did not differ between the transgenic and wild-type plants. These results provide new genetic evidence to support the view that FRK plays roles in regulating sugar and sorbitol metabolism in Rosaceae plants.]]> Mon 17 Jun 2019 12:58:51 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35061 2, respectively, in 0.5 M H₂SO₄ solution with a small Tafel slope of 43 mV/dec. Thus it outperforms many of the state-of-art molybdenum-based hydrogen evolution catalysts reported to date.]]> Mon 17 Jun 2019 12:09:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20652 Mon 14 Dec 2020 14:04:44 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50969 Mon 14 Aug 2023 15:18:37 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40592 Mon 08 Aug 2022 15:18:18 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41305 Mon 01 Aug 2022 12:23:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52807 Fri 27 Oct 2023 14:14:59 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39241 P = 0.022 adjusted for age, sex, and smoking). Cecal IELs were increased in IBS—diarrhea (relative risk ratio = 2.03, 95% confidence interval 1.13–3.63, P = 0.017). No difference was observed in alpha diversity of MaM or fecal microbiota based on IEL count. There was no difference in beta diversity of the MaM according to IEL count in the terminal ileal (TI) (P = 0.079). High TI IEL counts associated with a significant expansion of the genus Blautia (P = 0.024) and unclassified Clostridiales (P = 0.036) in colon MaM. Discussion: A modest but significant increase in IELs was observed in IBS vs. controls in a population-based setting. Subtle TI and cecal inflammation may play a pathogenic role in IBS but needs confirmation. Modest but discernible differences in the colonic MaM were seen according to TI IEL count but not IBS status.]]> Fri 27 May 2022 14:39:27 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49672 Fri 26 May 2023 15:35:47 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30277 Fri 21 Jun 2019 18:14:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42303 Fri 17 May 2024 16:00:48 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51712 Fri 15 Sep 2023 14:23:03 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54701 Fri 08 Mar 2024 12:07:53 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29190 p = 0.020), but not tMIP-ASPECT score (OR = 1.073, 95% CI = 0.820–1.405, p = 0.607), was independently associated with recanalization (AOL score of 2 and 3) at 24 hours after IVT. When recanalization was achieved, hemorrhagic transformation (HT) occurred more frequently in patients with slow collaterals (ATD ≥ 2.3 seconds) than those with rapid collaterals (ATD < 2.3 seconds) (88.9% vs 38.1%, p = 0.011). In conclusion, the velocity of collaterals related to recanalization, which may guide the decision-making of revascularization therapy in acute ischemic stroke.]]> Fri 01 Apr 2022 09:24:17 AEDT ]]>